By Salvatore A. E. Marras (auth.), Vladimir V. Didenko MD, PhD (eds.)
Although fluorescent probes and assays, which use power move (ET) for tracking DNA reactions, have extended lately, before there were no manuals summarizing the various varied protocols and probe designs. Fluorescent power move Nucleic Acid Probes: Designs and Protocols is the 1st book to supply the sort of assortment. within the quantity, hands-on experts-often the unique builders of the technique-comprehensively describe various fluorescent probes and units that use ET, together with molecular beacons, molecular holiday lighting fixtures, TaqMan® probes, LUX™ primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and good judgment gates for molecular-scale computation. Merging paintings on nanotechnology and fluorescent probes, the authors gather the 1st accomplished therapy of all of the key DNA- and RNA-based ET probes and current ideas for his or her optimized customized layout. They speak about either fluorescence resonance power move (FRET)-based and non-FRET-based constructs, and supply an entire set of innovations to watch DNA and RNA reactions, similar to hybridization, amplification, cleavage, folding, and institutions with proteins, different molecules, and steel ions. specific protocols are supplied for distance selection in protein-DNA complexes and the detection of topological DNA adjustments, mutations, and single-nucleotide polymorphisms. The protocols stick with the winning tools in Molecular Biology™ sequence layout, each one providing an overview of the foundations at the back of the approach, step by step precise directions, and entire troubleshooting.
Exhaustive and state of the art, Fluorescent strength move Nucleic Acid Probes: Designs and Protocols is a useful source for either beginner and skilled researchers who desire to use the newest advancements in fluorescent probes in any box of molecular biology.
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